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Nematode Sampling and Procedure

There is still time in October and November to take nematode samples for cotton, peanut, and soybeans.  Below is information on the procedures to follow.

Nematode Sampling Procedure

Properly taken samples from small field units can reduce production costs by allowing the grower to eliminate nematode control practices where they are not needed and implement control practices where they are needed. Improper sampling can lead to poor recommendations and economic losses which could have been avoided.

Both large areas and small areas should be sampled in a systemic, zigzag pattern.

As nematodes may not survive in upper 1-2 inches of soil due to extreme environmental conditions (hot and cold), first remove upper soil layer and then take samples using a 2.5cm (1-inch) diameter soil probe.

Take 20-30 soil cores in a zigzag pattern from regularly spaced locations throughout the field or area of concern (4-5 acre section).

Collected soil cores should be thoroughly mixed into one composite sample.

From each thoroughly mixed composite sample about 1 pint (1/2 liter) of soil should be transferred into a labeled plastic bag for nematode assay. Once sampled put in a zip-lock type bag and keep cool. Keep the sample out of direct sunlight preferably in a cooler. Label the plastic bags with sample number location and date of sampling. The assay forms are available at our office.

 The optimum time to take samples for nematode assay from various Georgia crops is given below: Crop  When to sample  Sampling depth in inches (cm)  Common Nematodes
Cotton Oct. and Nov. 8 inches (20) Lance, Reniform, Root-knot
Peanuts Sept. to Oct. 8 inches (20) Root-knot
Soybeans Sept. to Nov. 8 inches (20) Lance, Reniform, Root-knot, Cyst